The anticancer effect of acacetin and its induced RARγ-dependent AKT-p53 cross-talk were finally explored in vivo. We demonstrated that acacetin can strongly inhibit tumor growth and induce tumor shrink in mice (Fig. 6a), which is closely correlated with its increasing p53 expression accompanied by decreased RARγ and reduced AKT activity (Fig. 6b). This evidence concerns the gene TP53 and neoplasm.