In conclusion, our study demonstrated that miR-330-5p could act as a tumor suppressor for GBM cells by down-regulating the expressions of ITGA5 in both protein and mRNA levels, and either up-regulated miR-330-5p or down-regulated ITGA5 could reduce cell abilities of proliferation, invasion, and migration while promoting cell apoptosis. This evidence concerns the gene ITGA5 and neoplasm.