Among these, a putative interaction between PRUNE and tubulin, in particular β-tubulin, was most notable given previous studies highlighting the critical role of tubulins and microtubule-associated proteins during brain development, which may be mutated in autosomal recessive primary microcephaly (MCPH) (Woods et al., 2005; Bahi-Buisson et al., 2014; Sun and Hevner, 2014). Here, PRUNE1 is linked to autosomal recessive primary microcephaly.