PRUNE1 and neuroblastoma: We performed a cell-based microtubule-binding proteins spin-down assay (Darshan et al., 2011) with genetically modified SHSY5Y neuroblastoma cells to result in inducible clones overexpressing PRUNE wild-type or mutants, under a tetracycline inducible promoter (see ‘Material and methods’ section and Supplementary Fig. 3C).