Moreover, SL3261 co-infection led to loss of the M(IL-4) activation phenotype in NeMΦ, as measured by intracellular Relm-α expression, but induction of NOS2 was independent of previous M(IL-4) activation as indicated by equivalent expression in Relm-α positive and negative cells (Fig 3D & 3E). The gene discussed is NOS2; the disease is coinfection.