Parameters with gradual increment from BCLC stage 0 to stage D were serum bilirubin (p< 0.001), international normalized ratio (INR) (p< 0.001), serum alpha-fetoprotein (AFP) level (p< 0.001), platelet count (p< 0.001), and total tumor volume (p< 0.001). This evidence concerns the gene AFP and neoplasm.