TCN2 and Chagas disease: The present approach is based on parallel batches of distinct T. cruzi genotypes as target antigens, employing parasites strains of three more important genotypes associated with human infection and Chagas disease (TcI, TcVI and TcII) [4,38,59] although others genotypes exist such as TcIII [60–62], TcIV recently described as associated to oral transmission [63, 64] and TcV associated to the classical clinical forms of Chagas disease (cardiac and digestive) [39].