Knockdown of salusin-β with Ad-Salusin-shRNA dose- and time-relatedly reduced salusin-β, IL-1β, IL-6 and TNF-α mRNA levels, and NOX2 protein and 4-HNE levels in HG-treated H9c2 cells, reaching its maximal effects at the multiplicity of infection (MOI)=100 (Supplementary Figure 2) treated for 24 h (Supplementary Figure 3). This evidence concerns the gene IL1B and infection.