Although the percentages of CD8+ T cells on day 14 and 21 after the treatment and on day 26 after tumor inoculation from tumor-bearing mice were not significantly different, the mRNA level of IFN-γ in splenic CD8+ T cells on day 14 and 21 after the treatment was obviously higher than that on day 26 after tumor inoculation, suggesting that the function of CD8+ T cells was changed on day 14 and 21 after the treatment, though there was no increase in the CD8+ T-cell populations. Here, IFNG is linked to neoplasm.