The cell cycle assay showed that overexpression of CDC25A rescued the accumulation of cells in G1 phase induced by NPAS2 stable knockdown, whereas CDC25A silencing by siRNA increased the percentage of HCC cells in G1 phase induced by NPAS2 stable overexpression (Figure 6d and Supplementary Figure S4B). This evidence concerns the gene NPAS2 and hepatocellular carcinoma.