GCK and type 2 diabetes mellitus: We did not find significant differences in the mRNA expression of PDHA1 or GCK in the islets of controls vs T2D donors, although we saw a clear trend of reduced GCK mRNA expression in T2D islets (p = 0.07) which agrees with previous findings regarding the reduced GCK mRNA in a larger cohort of T2D islets30.