To clarify the role of NOX1 in colon cancer growth further, we utilized NOX1 shRNA in HT-29 human colon carcinoma cells to evaluate the effect of stable, silenced NOX1 expression on reactive oxygen production, tumor cell proliferation, cell cycle regulation, gene expression, signal transduction, and angiogenesis in both a cell culture model and in HT-29 xenografts. Here, NOX1 is linked to colon carcinoma.