NOX1 and neoplasm: The mRNA expression levels of genes related to cell proliferation, angiogenesis, and invasion were measured by real-time RT-PCR in HT-29 parental cells and in clonal variants expressing a scrambled shRNA sequence (SC) or Nox1 shRNA (6A) during logarithmic growth in cell culture or from tumor xenografts established in immuno-incompetent mice as described under “Experimental procedures.”