The peptide pool of multiple tumor antigens (Table 1) included ten TAAs, which are overexpressed in malignant cells from various cancers, including HCC such as survivin, NY-ESO-1, and carcino-embryonic antigen (CEA); two TAAs, alpha fetoprotein (AFP) and glypican-3 (GPC3), which are specifically overexpressed in cancerous hepatocytes from HCC; two antigens, HBV core antigen and HBV DNA polymerase, which are associated with chronic HBV infection. This evidence concerns the gene AFP and hepatocellular carcinoma.