To determine whether CoRL located in the intraglomerular compartment co-express podocyte proteins de novo in FSGS, double-staining was performed against the following four different podocyte proteins: podocin (Fig 4, membrane/cytoplasmic), nephrin (Fig 5, membrane/cytoplasmic), p57 (Fig 6, nuclear) and WT1 (Fig 7, nuclear). Here, NPHS2 is linked to focal segmental glomerulosclerosis.