To explore the molecular mechanisms by which Zbtb20 regulates hepatic lipogenesis, we first examined the expression of glycolytic and lipogenic enzyme genes in the liver from control and LZB20KO mice on the normal chow or a high-carbohydrate diet (HCD) rich in starch and sucrose (72.2% carbohydrates, 1% fat and 26.8% protein by energy), the latter enhances endogenous hepatic lipogenesis and chronically induces hepatic steatosis. This evidence concerns the gene ZBTB20 and Hepatic steatosis.