Also in colorectal HCT116 cells and ccRCC derived cell line (RCC-Shaw) the expression levels of miR-17-5p and miR-106b-5p were down-regulated compared to normal human kidney HK-2 cells, in conjunction with an increase of TRIM8 mRNA levels (Fig. 1c and d - Additional file 2: Figure S1b). Here, TRIM8 is linked to nonpapillary renal cell carcinoma.