Many genome editing techniques as ZFN and TALENs were performed to induce such beneficial modifications in CCR5 to eradicate HIV-1 infection but CRISPR/Cas9 has eclipsed all these because ZFN and TALENs requires much time and are expensive in doing target-specific genome engineering while CRISPR/Cas9 is relatively easy to use and needed a single-guided RNA to specify the target cleavage site and also provides on-target specificity. This evidence concerns the gene CCR5 and HIV-1 infection.