This further supports our analysis of HDAC effects and the use of HCT116 cell line as the latter has a perturbed epigenetic profile and a MSI phenotype, with a strong resistance to 5-FU therapy.31 Therefore, analysis of histone acetylation levels would help to provide better diagnostic and prognostic strategies in the future, while HDAC inhibitors are considered as the promising route for cancer treatment. This evidence concerns the gene HDAC9 and cancer.