Based on the situation that the expression and activity of AR has not been well characterized in neuroblastoma tumor cells and tumors, in our study, we investigated the effect of R1881 (a synthetic AR agonist; Patel et al., 2015) and two AR antagonists, MDV3100 and ARN-509, on cell viability, invasion, mobility and sphere-formation in the neuroblastoma cell lines SH-SY5Y (human neuroblastoma cell line) and Neuro-2a (mouse brain neuroblastoma, N2a), respectively. This evidence concerns the gene AR and neoplasm.