To further extend the observation that miRshRNA configuration has better downregulation activity than shRNA configuration of the matching sequence in RRV, we generated additional four matching pairs of siRNA sequences in shRNA and miRshRNA configurations targeted to human IDO-1, PDL1, and TGF-β2, key factors known to play an immune suppressive role in the tumor microenvironment. This evidence concerns the gene IDO1 and neoplasm.