To further elucidate the potential molecular mechanism through which TTF-1-promoter-operating miR-7 expression affected the growth of lung cancer cells, we used miRBase and TargetScan software to compare the downregulated genes in the p-T-miR-7 injection group and found 11 putative miR-7 target genes, including NXT2, C5orf22, PIGH, NDUFA4, TMEM97, CHAMP1, CNN3, LRRC8B, SAYSD1, TRMT13, and TPGS2 (Figure 4D), which also were closely related to tumor cell growth according to previous literatures.31, 32, 33, 34 Then, we verified the expression of these 11 predicted target genes, respectively. This evidence concerns the gene LRRC8B and lung cancer.