Finally, to confirm the effect of TTF-1-promoter-operating miR-7 expression on the growth and metastasis of tumor cells in vivo, we further detected the expression of cell-growth-related molecules including CDK2, CDK3, CDK4, and CDK6, as well as metastasis-related molecules including CXCR4, E-Cadherin, MMP2, MMP3, and MMP9, in tumor mass, respectively. This evidence concerns the gene MMP2 and neoplasm.