Interestingly, while withdrawal-induced anxiety-like behaviors emerge within the same period, between 8 h and 17 h following withdrawal (Matsumoto et al., 1993), withdrawal-induced anxiety-like behaviors in mice, which rise on the 1st day of withdrawal and last up to 21 days later, are positively correlated with the increase in EGR1-positive cells in the central amygdala and bed nucleus of the stria terminalis (Lee et al., 2015), which thus link the increase in EGR1 expression in the amygdala to the development of anxiety-like symptoms upon ethanol withdrawal. This evidence concerns the gene EGR1 and Anxiety.