As the results shown in Fig. 1a, comparing to the normal hepatic cell LO2 in which no HK-2 expression was detected, at least five HCC cell lines (HepG2, Hep3B, HCC-LM3, SMMC-7721, BEL-7402) expressed high levels of HK-2. The gene discussed is HK2; the disease is hepatocellular carcinoma.