This showed that neither EB1 nor EB3 knockdown affected the morphology of dynamic microtubules or prevented microtubules from extending to the cell periphery (Supplementary Figure S10) implying that the effects of EB3 knockdown on prostate cancer cell invasion are primarily due to loss of EB3 from microtubules and not secondarily due to changes in microtubule dynamics. This evidence concerns the gene MAPRE3 and prostate cancer.