It has been reported that knockdown of EB1 produced no change in microtubule dynamics in undifferentiated myoblasts (C2C12 cells)26 or CHO-K127 cells but shortened microtubules in Sertoli cells.28 EB3 knockdown produced curly microtubules in C2C12 cells26 but had no effect on dynamic microtubules in CHO-K1 cells.27 To test whether EB1 or EB3 knockdown affects microtubule dynamics in prostate cancer cell lines we assessed the morphology and distribution of dynamic microtubules in cells lacking EB1 or EB3 by labelling with an antibody against dynamic microtubules. This evidence concerns the gene MAPRE3 and Familial prostate cancer.