This shows that reduced CTCF gene expression disrupts endometrial cancer cell polarity, a morphological change characteristic of epithelial cancer development.34 There was no consistent change observed with CTCF knockdown compared to the three relevant controls in spheroid area, spheroid number, ZO-1 staining intensity or F-actin staining intensity (Supplementary Figure 2). The gene discussed is TJP1; the disease is endometrial cancer.