To establish that the Mad2l1 locus was in fact lost in tumor cells and that no RNA or protein was expressed, we analyzed DNA structure by genomic PCR (Figure 3A) and aCGH (Figure 3B), mRNA levels using qPCR (Figure 3C), and protein levels by Western blotting of tumor samples (Figure 3D). This evidence concerns the gene MAD2L1 and neoplasm.