The study has not only illustrated gene expression signatures of LIHC and related regulatory pathways and networks from the perspective of big data, featuring the cancers-associated pathway with top priority, ‘pathways in cancer,’ but also analyzed the diagnostic and prognostic values of four highlighted genes, namely BIRC5, E2F1, CCNE1 and CDKN2A, which were validated using Oncomine. Here, E2F1 is linked to cancer.