In an effort to expand our previous knowledge on the capacity of this mAb to activate such idiotypic network in apoE−/− mice, now we evaluated (i) the influence of age and gender in the induction of autologous anti-CS antibodies by chP3R99-LALA immunization, (ii) the effect of different doses of the mAb in the induction of the anti-idiotype antibody cascade, and (iii) the effect of the increase of chP3R99-LALA mAb dose in aortic atherosclerosis lesions of apoE−/− mice fed a HFHC diet. Here, APOE is linked to aortic atherosclerosis.