CD4 and medical procedure: By contrast, CD4+CD25hi T cells isolated from CsA- and BLT-treated patients showed reduced suppression even at high ratios of Tregs to CD4+ T cells (BLT = 19.52 ± 5.16; CsA = 17.74 ± 6.81; control = 51.03 ± 5.52 at 1:2 ratio; p < 0.01) and CD8+ T cells (BLT = 17.31 ± 4.83; CsA = 21.67 ± 3.20; control = 53.31 ± 5.66 at ratio 1:2 ratio; p < 0.01) (Figure 6), being associated with the reduced levels of FOXP3 and CD25 observed in the isolated CD4+CD25hi T cells of all kidney transplant patients (Figure S3B in Supplementary Material).