HLA-C and melanoma: Practically, this phenomenon seems to provide limited variability to the DNA barcode-based determination of T cell frequencies, resulting in a very tight correlation with T cell frequencies determined by combinatorially encoded fluorescently labeled MHC multimers [r2 = 0.967, across 10 healthy donors (32 responses) and r2 = 0.901 across 11 melanoma patients (15 responses)] [28].