AKT1 and glioblastoma: Furthermore, miR-UL112-3p mimics/inhibitor and plasmid expressing constitutively active p-AKT (CA-AKT)/dominant negative p-AKT mutant (DN-AKT) were co-transfected in GBM cells, and the corresponding changes in p-AKT expression were verified by immunoblotting (Fig. 7A).