In our previous study, we have screened 24 BBZs for their antibacterial activity against water borne and clinical E. coli isolates collected from UTI patient samples and observed six compounds namely PPEF, PPVF, NNEF, PYRVF, PYMVF and EPEF structures depicted in (Fig. 7) to be the most potent17. The gene discussed is PPEF1; the disease is bacterial urinary tract infection.