CEACAM8 and Alzheimer disease: We also used (i) hematoxylin and eosin staining to confirm the presence of polysegmented nuclei in cells that have migrated perivascularly or within the parenchyma (8), (ii) napthol AS-D chloroacetate esterase staining in brain sections to confirm the presence of cells of the granulocytic lineage specifically in AD brains (43, 44), and (iii) staining for the neutrophil-specific marker, CD66b, which likewise confirmed that neutrophils were present specifically in the brains of AD subjects but not age-matched controls (8).