The analysis of cells gated on the CD15– lymphoid fraction showed that after 2 weeks of differentiation CHIR990921-induced HE developed a much larger population of CD56+ NK cells (~25%) compared to OP9-induced cells (~15%), and the fraction of CD56+CD16+ double-positive NK cells, the major population subset of NK cells at this stage of development, was small for both CHIR990921 and OP9-induced cells (Fig. 2g). The gene discussed is NCAM1; the disease is hereditary elliptocytosis.