In vivo expression of DNMT3A led to hypermethylation in the genome.23, 24 Coincidentally, the anergy of tumor-suppressor function of RB1 was also highly associated with the methylation in the promoter region in multiple human malignant tumors, including HCC.25, 26 After analyzing the correlation between the expression of Linc00441 and the methylation rate of RB1 in HCC samples from matched patients, we found a positive correlation between two parameters (Figure 7a). Here, RB1 is linked to cancer.