Although generally considered to be cytosolic, a significant proportion of tau is associated with plasma membranes.39, 40 Membrane-associated tau is predominantly dephosphorylated at serine and threonine residues.40, 41 As some of the vesicles (ectosomes, exosomes) associated with tau release originate at the plasma membrane, it was of interest to investigate the proportions of tau at membranes in wild-type and 3xTg-AD slices to determine whether there is any association with tau release. The gene discussed is MAPT; the disease is Alzheimer disease.