MAPT and Alzheimer disease: When stimulated with KCl, wild-type slice cultures showed significant increases in tau release compared with non-stimulated wild-type slice cultures (Figures 4c, P<0.05), in agreement with published data from dissociated rodent cells11 and in vivo in wild-type mice.12 In contrast, 3xTg-AD slice cultures stimulated with KCl did not demonstrate any further release of tau when compared with non-stimulated 3xTg-AD slice cultures (Figure 4d).