Interestingly, as revealed by combined index sorting of IGRP265-273 multimer-positive CD8+ T cells and TCR sequencing for T1D-1, those cells that expressed the dominant TCR α-chain detected in T1D-1 and T1D-3 (IGRP α1; depicted as red piece of pie charts in Fig. 1c) had the highest median fluorescence intensity in the IGRP265-273-specific multimer staining (Fig. 1d) as compared to the IGRP α2-containing cells (depicted as yellow piece of pie charts in Fig. 1c; P < 0.05) and cells with other TCR α-chains (P < 0.001). Here, CD8A is linked to type 1 diabetes mellitus.