CD8A and type 1 diabetes mellitus: We first applied next generation sequencing of the TCR α-chain repertoire to determine whether the approach could detect the dominant IGRP265-273-specific chains IGRP α1 and IGRP α2 in bulk CD8+ T cells from the original patients T1D-1, T1D-2, and T1D-3 (Supplementary Fig. S4).