FOXO1 and metabolic dysfunction-associated steatotic liver disease: To better understand the gene (notably FOXOs)-environment (different types of diets) interaction in the development of NAFLD, we set out to characterize wildtype (WT) and Foxo1/3/4 liver-specific triple knockout mice (LTKO) that were fed either regular chow, simply high-fat or moderately high-fat plus cholesterol diet.