NEDD4 and neoplasm: Notably, we found that in vitro tumour sphere numbers and Aldh+ cell population were reduced upon glucose depletion, NEDD4 knockdown, NEDD4 tyrosine phosphorylation-deficient mutation, GCN5 knockdown, H3.3 knockdown and deficiency of H3 ubiquitination (Fig. 6c–h and Supplementary Fig. 9a–l).