In summary, our new findings provided evidence that JPJD could inhibit TGF-β-induced EMT in CRC through TGF-β/Smad2/3 mediated Snail/E-cadherin expression, and this might be the potential effect mechanism of JPJD on inhibiting the invasion and metastases of CRC in vitro and in vivo. Here, SMAD2 is linked to colorectal carcinoma.