Transcript levels of Hif1a were however not affected either by maternal obesity or the exercise intervention (Control 1.0 ± 0.06, Obese 1.02 ± 0.15, Obese-Ex 0.94 ± 0.09; relative expression), with male only expression also being similarly unaffected (Control, 1.0 ± 0.07, Obese 0.92 ± 0.24; Obese-Ex, 0.97 ± 0.07), implying that the observed changes in HIF1A protein levels were not a result of increased transcription at this locus. The gene discussed is HIF1A; the disease is obesity due to melanocortin 4 receptor deficiency.