Apart from Helios, the percentage of PD1+ Foxp3+ CD4+ cells in ovarian cancer of RORγt−/− mice (42.4±2.97% (control) and 24.55±4.89 (RORγt−/−), n=5 per group) is significantly lower (Fig. 2c). The gene discussed is FOXP3; the disease is ovarian cancer.