Target genes of the continuously overexpressed and underexpressed miRNAs during CRC development (without miR-378 homologues) (Fig. 2) participate in pathways related to signal transduction (PI3K-Akt KEGG pathway ID: hsa04151, MAPK-KEGG pathway ID: hsa04010) and cancer (KEGG pathway ID:hsa05200) from the top 24 selected KEGG pathways. The gene discussed is AKT1; the disease is colorectal carcinoma.