NLRP3 and type 1 diabetes mellitus: To investigate the role of the NLRP3 inflammasome in the pathogenesis of T1D, C57BL/6 wild-type (WT) male mice were inoculated intraperitoneally with MLD-STZ (40 mg/kg) for five consecutive days and were assessed for mRNA expression of NLRP3, ASC and pro-IL-β in the PLNs at 7 and 15 days after starting the STZ injections.