To determine if SHMT1 loss-mediated downregulation of IL-6 and IL-8 also occurs in other ovarian cancer cell lines, we measured IL-6 and IL-8 mRNA levels in other ovarian cancer cell lines COV504 and COV413B expressing SHMT1 shRNA and observed similar results (Figures 5b and c). This evidence concerns the gene SHMT1 and ovarian cancer.