SMC4 and neoplasm: Furthermore, the three-dimensional spheroid invasion assay, which is considered to better mimic in vivo tumor invasion,24 revealed that SMC4-transduced SW1088 cells that had been cultured in Matrigel for 10 days displayed morphologies typical of highly aggressive invasiveness, with nearly all individual cells presenting more outward projections as opposed to the vector-transduced control cells (Figure 4c, top).