Meanwhile, we found that the phosphorylated levels of TGFBR1, Smad2 and Smad3 and the nuclear levels of Smad2/Smad3 were markedly increased in the SMC4-overexpressing glioma cells but decreased in the SMC4-silenced cells (Figures 6e–f). The gene discussed is SMAD3; the disease is glioma.