In agreement with this hypothesis, overexpressing FAM83A in pancreatic cancer cells markedly increased the CD133+ population (Figures 2b and c), which is exclusively tumorigenic and highly chemoresistant.12 Moreover, FAM83A-transduced cells formed significantly larger and higher numbers of spheres in the tumorsphere formation assay compared with vector control cells (Figure 2d). This evidence concerns the gene SACK1A and familial pancreatic carcinoma.