APP and Alzheimer disease: After treatment, BN-PAGE followed by Western blot with human Aβ/CTF antibody 82E1 and Aβ/APP antibody 6E10 revealed the added human Aβ as a band at 240 kDa, corresponding to the molecular weight of APP, only in the APP overexpressing AD transgenic neurons but not the wild-type neurons (Supplementary Fig. 6a).