Our results show that the cytotoxicity, exhibited by CART-HER2 cells against HER2-positive GC cells and HER2-knockdown cells, was significantly higher than that exhibited by NT T cells; this rules out the influence of the vector system on our results and suggests that CAR-T cells contacted the target cells with high affinity and lysed them in an MHC-independent manner. This evidence concerns the gene ERBB2 and gastric cancer.