Mutations on SQSTM1 are known to associate with ALS/FTD; In this research, SQSTM1 was categorized in ALS+FTD group, but it also involved in some ALS without FTD cases[48], which directly validated our algorithm and highlighted the importance of SQSTM1/p62 in pathology across ALS-FTD spectrum. This evidence concerns the gene SQSTM1 and amyotrophic lateral sclerosis.