Productive infection by two additional ZIKV strains, Puerto Rico 2015 (PRVABC59) and Thailand 201326, was observed in primary HESC by a plaque forming assay (PFA) (Supplementary Fig. S2a) 3 days post-infection, with intracellular co-localization of the E protein and dsRNA with calreticulin and vimentin, respectively (Supplementary Fig. S2b,c). Here, VIM is linked to infection.